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Evaluation of hemp cultivars for CBD production in 2019

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Authors:

George Stack, Jacob Toth, Craig Carlson, Mariana Marrero Gonzàlez, Rebecca Wilk, Jamie Crawford, Ali Cala, Glenn Philippe, Jocelyn Rose, Don Viands, Chris Smart, Larry Smart


Production of cannabinoids:

Chemical diagram showing how CBD and THC are derived from cannabigerolic acid

Cannabidiol (CBD) is becoming very popular as a supplement for relief of seizures, pain, and anxiety. It is made in specialized leaf hairs on female floral tissue.

Locations and production methods:

Chart showing the average percent of plot flowering during each week of our trials
Chart showing the average percent of plot flowering during each week of our trials

We are testing cultivars in two small-plot trials on Cornell research farms in Geneva and Ithaca. Plants were established from seeds or cuttings in 50-cell flats in early May. Females were transplanted to raised beds with plastic mulch and drip irrigation on June 5 (Geneva) and June 7 (Ithaca).

Plant trait measurement:

We are measuring several plant traits throughout the growing season including plant height, time to flowering, flowering form, pest and disease incidence, chlorophyll content, and leaf area. We saw variability within and between cultivars for flowering time and form. The ‘Kayagene’ varieties were flowering when we planted them and most ‘First Light’ varieties haven’t flowered yet.

Harvest yield and quality assessment:

We will harvest whole plants and air dry them to measure dry biomass. Cannabinoids will be measured from floral tissue to determine regulatory levels (top 10 cm) and whole plant yields.

Cultivars/lines in our 2019 trials:

Cultivar/Line Source Cutting or Seed Germ. % Female
AC/DC Winterfox Farms, Eagle Point, OR Seed (Dioecious) 97% 49%
Otto II Winterfox Farms, Eagle Point, OR Seed (Dioecious) 93% 48%
Nebraska Feral Winterfox Farms, Eagle Point, OR Seed (Dioecious) 46% 50%
A2R4 Winterfox Farms, Eagle Point, OR Seed (Dioecious) 68% 52%
RNF JDMI Limited, Talent, OR Seed (Dioecious) 85% 53%
RN13a Go Farm Hemp, OR Seed (Dioecious) 73% 40%
RN16 Go Farm Hemp, OR Seed (Dioecious) 98% 51%
RN17 Go Farm Hemp, OR Seed (Dioecious) 97% 59%
RN19 Go Farm Hemp, OR Seed (Dioecious) 97% 55%
Brilliance Green Lynx Farms, CO Seed (Feminized) 90%  
KG9201 Kayagene, CA Seed (Feminized) >90%  
KG9202 Kayagene, CA Seed (Feminized) >90%  
T2 Boring Hemp, CO Seed (Feminized) 100%  
TJ’s CBD Stem Holdings, OR Cutting    
Tangerine NY Hemp Source, NY Cutting    
Late Sue NY Hemp Source, NY Cutting    
Deschutes F1 Industrial Seed Innovations, OR Seed (Feminized) 96%  
Umpqua F1 Industrial Seed Innovations, OR Seed (Feminized) 94%  
Rogue F1 Industrial Seed Innovations, OR Seed (Feminized) 100%  
First Light 49 Sunrise Genetics, CO Cutting    
First Light 58 Sunrise Genetics, CO Cutting    
First Light 70 Sunrise Genetics, CO Cutting    
First Light 71 Sunrise Genetics, CO Cutting    
First Light 80 Sunrise Genetics, CO Cutting    
First Light 70×70 Sunrise Genetics, CO Seed (Feminized) 62%  
First Light 71×71 Sunrise Genetics, CO Seed (Feminized) 96%  
Cherry 5 HempLogic, WA Seed (Dioecious) 83% 46%
Cherry 307 HempLogic, WA Seed (Dioecious) 45% 52%
Cherry 308 HempLogic, WA Seed (Dioecious) 88% 42%
NY Cherry Genesis Hemp Alliance, NY Seed (Dioecious) 69% 34%
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